QUALITATIVE ANALYSIS

 

Name of teacher:           

Week:                                                                   Date:                                                     Time:

Period:                                 Duration: 1 HR 20 MIN.                                                  Average age of learners: 16YEARS

Subject:                               CHEMISTRY                                                                         Class: SS THREE

Topic:                                    QUALITATIVE ANALYSIS

Sub topic:

Reference materials:

(1) ESSENTIAL CHEMISTRY, TONALD PUBLISHERS, I. O ODESINA

(2) NEW SCHOOL CHEMISTRY, AFRICAN FIRST PUBLISHERS, OSEI YAW ABABIO

(3) INTERNET

Instructional materials: beaker, beam balance

Entry behavior: The students have been taught density.

Objective: At the end of the lesson the students should be able to:

1.       Identify salt with their appearance

2.       Identify ions with flame test

3.       Explain test for gases

4.       State the reagents used in test for cations and anions.

5.       Explain test for organic compound.

CONTENT

QUALITATIVES ANALYSIS

In qualitative analysis, we identify the elements and compounds that are present in a sample of a given substances.

The preliminary test we carried out under the following headings:

1.       Appearance of the compound (colour)  2.  Flame Test      3. Action of Heat (Identification of gases).

APPEARANCE

PROBABLE SALT / COMPOUND

Green

Iron(ii) salts, Iron (iii) salt are usually pale green

Yellow/ brown

Lead(ii)oxide, iron(iii) salt solution, candium sulphide yellow

Black or red

CuO, PbS, CuS, Ag2S, HgS

White colourless

Ca2+, Pb2+, NH4+, Na+, Al3+, Zn2+ salts and ZnS, MnS

Smell of NH3

Ammonium salt

Smell of sulphur

Trioxosulphate (iv) salts

Smell of H2S

Sulphides

Deliquescent

Chloride or trioxonitrate (v)

FLAME TEST

COLOUR FROM FLAME TEST

PROBABLY ION

Brick red

Ca2+

Deep green

Cu2+

Blue

Pb2+

Persistence golden yellow

Na+

Persistence lilac

K+

Persistence light green

Ba2+

GASES EVOLVED ON HEATING

GAS

INFERENCE

NO2

NO3-

CO2

CO32- OR HCO3-

O2

NO3-

SO2

SO32-

NH3

NH4+

ACTION OF HEAT ON SPECIMEN

OBSERVATION

INFERENCE

White when cold and yellow when hot

Zn2+ (ZnO) present

Yellow when cold and reddish brown when hot

Pb2+ (PbO)

Reddish-brown

Fe3+ (Fe2O3)

Water vapour which condenses at the upper part of the tube

Hydrated salts HCO3- or OH- present.

White sublimate

Ammonium salt

TEST FOR GASES

GAS

COLOUR/SMELL

TEST

RESULT IF POSITIVE

Cl2

Greenish-yellow, pungent

a. Moist blue / litmus paper.

b. Bubble through bromine solution

Turns red then bleaches it (acidic)

Bromine is liberated and solution turns yellow or orange.

NO2

Reddish-brown, pungent

a. Moist blue litmus paper

b. Bubble through fresh FeSO4 solution

Turn red (acidic)

Solution turns blue

NH3

Colourless pungents smell like that of urine

a. moist red litmus paper

b. bring in contact with drop of conc. HCl on a glass rod.

Turns blue (alkaline gas)

White dense fumes due to the presence of NH4Cl

H2S

Colourless, rotten egg smell

Moist lead(ii) ethanoate paper

Turns black

HCl

Colourless

a. damp blue litmus paper.

b. blows across mouth of test tube

c. bring in contact with drop of NH3 solution on a glass rod

Turns red (acidic)

Copious fuming

White dense fume of ammonium chloride.

CO2

Colourless, odourless

 

a. damp blue litmus paper

b. bubble through lime water in excess of it.

Turn red (acidic)

Turns milky (ppt of CaCO3) milky colour disappears due to formation of CaHCO3

H2

Colourless, odourless

a. damp blue and red litmus paper

b. lighted splint

No effect (neutral gas)

Little explosion occurs giving pop sound; burns with blue flame, it mixed with air

O2

Colourless, odourless

a. damp blue and red litmus paper

No change (neutral)

H2O

Colourless, odourless

a. damp blue or red litmus paper

b. anhydrous CuSO4 (white in colour)

No change (neutral gas)

It turns to blue crystal of CuSO4.5H2O

SO2

Colourless, pungent smell

a. damp blue litmus paper

b. bubble through K2Cr2O7 acidified with dilute H2SO4

c. bubble through KMnO4 solution acidified

Turn red (acidic)

Turn from orange to green

 

Turn from purple to colourless (reducing agent)

Note: H2S gives the same result with K2Cr2O7 and KMnO4 all acidified but there will be yellow deposit of sulphur.

TEST FOR CATION

Cation   Reaction with

cation

Dil.HCl

Dil. H2SO4

Dil. NaOH

Dil. NH3

NH4Cl + Dil NH3

H2S

H2S/Dil HCl

Pb2+

White crystalline ppt dissolving when warmed, reappears on cooling

White powdered ppt

White powdered ppt soluble in excess

White powdered ppt insoluble in excess

White crystalline ppt on adding NH4Cl

Black ppt

Black ppt

Al3+

No ppt

No ppt

White gelatinous ppt soluble in excess reagent

White gelatinous ppt insoluble in excess reagent

White gelatinous ppt.

No ppt

No ppt

Zn2+

No ppt

No ppt

White gelatinous ppt soluble in excess reagent

White gelatinous ppt soluble in excess reagent

No ppt

White ppt

No ppt

Ca2+

No ppt

No ppt (may leave slightly turbidity)

White powdered ppt insoluble in excess NaOH

No ppt turbidity due to CO3 in reagent

No ppt

No ppt

No ppt

Fe2+

No ppt

No ppt

Dirty green gelatinous ppt insoluble in excess reagent

Dirty green gelatinous ppt insoluble in excess reagent

Dirty green gelatinous ppt.

Slight ppt or non

No ppt

Fe3+

No ppt

No ppt

Reddish- brown gelatinous ppt insoluble in excess reagent

Reddish-brown gelatinous ppt insoluble in excess reagent

Reddish-brown gelatinous ppt.

Yellow to pale-green with yellow deposit

Yellow to pale-green with yellow deposit

Cu2+

No ppt

No ppt

Light-blue gelatinous ppt. in excess reagent

Light blue gelatinous ppt soluble in excess giving deep blue solution.

Light blue  ppt which dissolves in excess giving a deep blue solution

Black ppt

Black ppt

CONFIRMATION TEST FOR CATIONS

TEST

OBSERVATION

INFERENCE

1. To the aqueous solution, add (NH4)2CO3 or ammonium oxalate

White ppt

Ca2+ confirmed

2. To an aqueous solution is added NH3 sol. And NH4Cl solution

White ppt

Al3+ confirmed

3. a. To an aqueous solution add K2Cr2O4

    b. solution KI

Yellow ppt

Yellow ppt

Pb2+ present

Pb2+ present

4. a. To the solution add potassium hexacyanoferrate(ii) solution K4Fe(CN)6 aq

     b. To the solution add potassium hexacyanoferrate(iii) solution K3Fe(CN)6 aq

Light blue ppt

 

Deep blue ppt

Fe2+ present

 

Fe2+ present

5. a. To the specimen solution add K4Fe(CN)6

   b.   To the specimen solution add thiocyannate solution KSCN or ammonium thiocyanate solution.

Deep blue ppt.

Deep blood red colouration

Fe3+ present.

Fe3+ present.

6. specimen solution + potassium hexacyanoferrate (ii) solution K4Fe(CN)6

Brown ppt

Cu2+ present.

7. a. To the solution add (NH4)2S solution or H2S

    b. To the solution add K4Fe(CN)6

Dirty white ppt.

White ppt.

Zn2+ present.

Zn2+ present.

8. warm solution gently with slightly excess of NaOH aq

Gas evolved turned red litmus blue.

NH4+ present.

TEST FOR ANION

TEST

OBSERVATION

INFERENCE

1. a. To the solution add AgNO3 + dil. NH4OH in excess

     b. Salt solution + HNO3 + AgNO3 to the mixture in (a) above + NH4OH in excess.

     c. To a salt sample add MnO2 and Conc. H2SO4 and warm gently

White ppt.

White ppt dissolves.

White ppt. insoluble in NHO3, white ppt dissolves.

Greenish yellow gas with suffocation odour and turns damp blue litmus paper red  finally bleaches it.

Cl- present.

Cl- confirmed.

Cl- present.

Cl- present.

Cl- present. The gas is Cl2(g)

2. To the salt solution add dil. HCl or Conc. H2SO4 and warm gently.

Greenish yellow gas with suffocation odour and turns dump blue litmus paper red and finally bleaches it.

The gas is CO2. CO32- or HCO3- present.

3. To the salt solution add BaCl2 solution and then add few drops of hydrogen chloride solution.

                     Or

Salt solution + Ba(NO3)aq + dil. HNO3

 

Salt solution + acidified dil. K2Cr2O7

 

To about 2cm3 of the clear solution add about 3cm3 of freshly prepared FeSO4, then add about 3cm3 of Conc. H2SO4 slowly down the side of the test tube held in a slanting position.

                    Or

Salt solution + Conc. H2SO4

White ppt. which does not dissolve in presence of HCl acid.

White ppt. soluble in excess dil. HCl

 

White ppt. insoluble in dil. HNO3

White ppt. soluble in dil. HNO3.

The orange colour of K2Cr2O7 turns green.

A brown ring is formed at the junction of the two layers.

 

 

 

 

Reddish-brown fumes evolved

SO42- present.

 

SO32- present.

 

SO42- present.

SO32- present.

SO32- present.

 

NO3- confirmed, the brown ring is due to the formation of FeSO4.

 

 

 

NO, NO3- present.

TEST FOR ORGANIC COMPOUND

TEST

OBSERVATION

INFERENCE

1. TEST FOR STARCH (C5H10O5)n

a. Into a test-tube containing suspected starch solution, add iodine solution (KI/I2).

b. In a test-tube containing suspected starch solution, add iodine solution, warm and cool.

 

Blue- black colouration observed.

 

Blue-black colouration disappeared when warmed and reappeared on cooling.

 

Starch confirmed.

 

Starch confirmed.

2. TEST FOR REDUCING SUGAR

(GLUCOSE AND FRUCTOSE)

a. Into a test-tube containing suspected reducing sugar solution, add Fehling’s solution and warm in water bath.

 

 

Red precipitate observed

 

 

Reducing sugar confirmed.

3. TEST FOR NON-REDUCING SUGAR

(COMPLEX SUGAR)

a. Into a test-tube containing suspected non-reducing sugar solution add dil. HCl and warm for 3-5 minutes. Now cool the tube; neutralize the excess acid, with NaOH or Na2CO3 solution (test with litmus paper). Now add few drop of Fehling solution and warm in water bath.

 

 

Red precipitate observed

 

 

Non-reducing sugar confirmed.

4. TEST FOR PROTEINS

a. To a solution or substance, suspected to be protein, add soda lime solution, heat the mixture in boiling tube.

 

b. To a solution or substance suspected to be protein, add 3cm3 of NaOH sol. Then add 1-2 drops of CuSO4

 

A gas is given off having pungent smell, turn red litmus paper blue and form dense white fumes with drop of HCl solution. The gas is NH3.

A purple colour observed

 

Protein is confirmed.

 

 

 

Protein is confirmed.

5. TEST FOR FATS AND OILS

a. Press substance suspected to be fats and oils on a filter paper or plain sheet of paper

 

b. Add a few drops of liquid suspected to be oil on a filter  paper or plain sheet

 

c. Add equal amount of 5ml of NaOH in a liquid suspected to be oil or solid substance suspected to be fat. Heat while stirring or shaking the test-tube. Precipitate is filtered. Residue + distilled water, shake.

 

The area of the paper turns translucent i.e light compass through it.

The region where the liquid touches diffuses and turns the paper translucent.

Oil turns cloudy on addition of reagent, on heating, a mass of white ppt is produced which could be a product of saponification. A lather is produced indicating the white ppt is a soap

 

Fats or Oil confirmed.

 

 

Oil is confirmed.

 

 

Oil is confirmed.

 

PRESENTATION

Step I: The teacher explains how to identify salt with their appearance.

Step II: The teacher explains test for ions with flames.

Step III: The students chorus test for ion using flame test.

Step IV: The teacher leads the students to test for cations and anions.

Step V: The teacher explains test for organic compound.

Step V: The teacher allows the students to ask questions.

EVALUATION

The teacher asks the following questions:

1.       Identify salt with their appearance

2.       Identify ions with flame test

3.       Explain test for gases

4.       State the reagents used in test for cations and anions.

5.       Explain test for organic compound.

ASSIGNMENT

1a.       Give one chemical test to distinguish between CH3CH2OH and CH3COOH.

b.         State what is observed when aqueous ammonia is added to :

            i. litmus paper

ii. Pb(NO3)2 solution in drops until in excess

            iii. Freshly precipitated AgCl in excess.

            b.   State what would be observed: I. if a fresh precipitate of silver chloride was

(i) expose to light

 (ii) shake with excess aqueous ammonia.

II.   On bubbling chlorine through a solution of potassium bromide.

C.         A given crystalline solid is suspected to be either sodium chloride or ammonium tetraoxosulphate (vi). Describe how you would  use the indicator to identify the solid.

2a.       When a drop of concentrated trioxonitrate (v) acid was added to potassium iodide solution, a violet-coloured gas was evolved.

i.                    What is the name of the gas evolved

ii.         State the functions of concentrated trioxonitrate (v) acid in the reaction.

iii.        What would be observed if starch solution were added to the reaction mixture?

            b.   I. What technique would you use to purify a sample of sodium chloride contaminated with ammonium chloride?

            II. Given sodium hydroxide solution, outline the procedure you would use to determine whether or not all the ammonium chloride in b(I) above had be removed.

           

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